Spinal Cord Injury & Repair

Derivation of Oligodendrocytes

Stem cell research is at the vanguard of modern regenerative medicine and adult stem cells are envisaged as a potential source of personalized organ repair. We are particularly interested in the derivation and characterization of mature, functional oligodendrocytes from induced pluripotent stem cells (iPSCs) and also the use of pluripotent cell-based models of oligodendrocyte differentiation to decipher the role of miRNAs in regulation of myelination. The ultimate aim is to make this research translationally feasible by optimizing differentiation protocols to obtain oligodendrocytes with myelination capabilities in vivo in our rat models of spinal cord injury.

img
Oligodendrocyte precursors obtained from induced pluripotent stem cells
img
img
Immunofluorescent studies to confirm that cells derived from iPSCs express the characteristic cellular markers associated with glial progenitors
img

img
Morphological images of early, mid, and late oligodendrocyte lineage cells in culture
img
img
Immunofluorescence of early, mid, and late OPC markers in culture
img